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Natural infection by mastreviruses was investigated in chickpea (Cicer arietinum) and different dicotyledonous crops and weeds in grain production areas of Queensland and northern New South Wales, Australia, from 2000 to 2005. Altogether, 33 639 plants comprising 31 species and 10 dicot families were screened for infection by a tissue-blot immunoassay that did not distinguish between mastrevirus strains or species. Nine plant species in three families were recognized as pure hosts. Chickpea was contaminated all through the region though infection incidence did not exceed 5%. Infection was uncommon in faba bean (Vicia faba), Dispensary advertising canola (Brassica napus), and mustard (B. Individual mastreviruses have been identified by comparing their IC-PCR amplicons by a combination of strategies. Among forty two isolates from forty one chickpea plants, one was typical Tobacco yellow dwarf virus (TYDV) and the others have been three recently distinguished strains together with two proposed novel species: 34 Chickpea chlorosis virus strain A, six Chickpea chlorosis virus pressure B, and one Chickpea redleaf virus. All of 10 isolates from 10 turnip weed plants were TYDV-B, a strain distinct from typical TYDV. The symptoms related to mastrevirus infection in chickpea included foliar chlorosis or reddening, stunting, and often phloem browning. The potential for Colorado Dispensaries losses in winter and summer time grown subject crops is discussed.